Patterning of Nano-Hydroxyapatite onto SiO2 and Electro-spun Mat Surfaces Using Dip-Pen Nanolithography

• Report formulation of nanohydroxyapatite (nanoHA) based ink for dip pen lithography (DPN). 30-50 % (wt/Vol) Glycerol in isopropyl alcohol is necessary to achieve the required viscosity 5-15 cP printing nanoHA (3 w/v %)-formulated ink. Ultra sonication glycerol formulated aided disperse well nanoparticles get ~200 nm size and helped controlled The optimal consisted 30% 3 with printed features ranging from 745 – 1175 on a SiO2 substrate. development DPN patterning onto electrospun mat will enable study single cellular interactions nanoscale surfaces, including nanoHA-gradient-scaffold interface tissue engineering. We have demonstrated ability controlled-pattern hydroxyapatite SiO 2 surfaces using dip-pen nanolithography One keys success this research was inks well-defined physicochemical properties specific use nanolithography. Studies effect terms dispersion, stability, accuracy determined as 30-50% alcohol. Dynamic light scattering (DLS) scanning electron microscopy (SEM) were used determine dispersion solution. solution yielded dispersed particles stability evaluated by zeta-potential measurements turbidity (the tendency sedimentation over time) showed higher (30-50% against 0% glycerol). Atomic force (AFM) SEM utilized image patterns substrate an mat. Additionally, scaffold without fiber damage soft-matter. many